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10X Genomics chromium controller
Chromium Controller, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/controller/chromium+controller/nct05030805-2-10-12
Average 90 stars, based on 1 article reviews
chromium controller - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Single Cell:

Article Title: Pathogenic IgE-fated memory B cell responses retain functional plasticity.
Article Snippet: Longlived immunoglobulin E (IgE) responses against innocuous environmental and dietary antigens (Ags) are maintained by an IgG1dominant memory B cell (MBC) compartment primed for interleukin4 (IL4) responsiveness.. The plasticity of the MBC compartment destined for IgE class switch recombination remains poorly understood.. In this work, we report critical IL4 and IL13 dependency for the pathogenic IgE fate of type 2–polarized MBCs in allergy.

Article Title: Pathogenic IgE-fated B cell memory retains functional plasticity
Article Snippet: .. Single-cell 5’ gene expression and V(D)J libraries were prepared with Chromium Single-Cell V3 Reagent Kit and Controller (10x Genomics; Pleasanton, CA). .. Libraries were sequenced with NextSeq2000 (Illumina; San Diego, CA).

Article Title: MICROGLIA AND INFILTRATING T-CELLS ADOPT LONG-TERM, AGE-SPECIFIC, TRANSCRIPTIONAL CHANGES AFTER TRAUMATIC BRAIN INJURY IN MICE
Article Snippet: .. The concentration and viability (>90%)were confirmed using a K2 Cellometer (NexcelomBioscience LLC, Lawrence,MA)withAO/PI reagent, and ~5,000–10,000 cells were loaded on 10x Genomics Chip A using Chromium Single Cell V3 Reagent Kit and Controller (10x Genomics, Pleasanton, CA). .. Single-cell 3′ RNA-Seq libraries were prepared according to the manufacturer protocol (10x Genomics, Pleasanton, CA).

Article Title: Crosstalk between nonclassical monocytes and alveolar macrophages mediates transplant ischemia-reperfusion injury through classical monocyte recruitment.
Article Snippet: Cells were counted using acridine orange/propidium (AO/PI) and Cellometer K2 (Nexcelom), and cell viability exceeded 90%. .. Single cell 3’ RNA-Seq libraries were prepared using Chromium Single Cell V2 Reagent Kit and Controller (10x Genomics). .. Libraries were assessed for quality (TapeStation 4200, Agilent Technologies) and then sequenced on NextSeq 500 or HiSeq 4000 instruments (Illumina).

Gene Expression:

Article Title: Pathogenic IgE-fated memory B cell responses retain functional plasticity.
Article Snippet: Longlived immunoglobulin E (IgE) responses against innocuous environmental and dietary antigens (Ags) are maintained by an IgG1dominant memory B cell (MBC) compartment primed for interleukin4 (IL4) responsiveness.. The plasticity of the MBC compartment destined for IgE class switch recombination remains poorly understood.. In this work, we report critical IL4 and IL13 dependency for the pathogenic IgE fate of type 2–polarized MBCs in allergy.

Article Title: Pathogenic IgE-fated B cell memory retains functional plasticity
Article Snippet: .. Single-cell 5’ gene expression and V(D)J libraries were prepared with Chromium Single-Cell V3 Reagent Kit and Controller (10x Genomics; Pleasanton, CA). .. Libraries were sequenced with NextSeq2000 (Illumina; San Diego, CA).

Concentration Assay:

Article Title: MICROGLIA AND INFILTRATING T-CELLS ADOPT LONG-TERM, AGE-SPECIFIC, TRANSCRIPTIONAL CHANGES AFTER TRAUMATIC BRAIN INJURY IN MICE
Article Snippet: .. The concentration and viability (>90%)were confirmed using a K2 Cellometer (NexcelomBioscience LLC, Lawrence,MA)withAO/PI reagent, and ~5,000–10,000 cells were loaded on 10x Genomics Chip A using Chromium Single Cell V3 Reagent Kit and Controller (10x Genomics, Pleasanton, CA). .. Single-cell 3′ RNA-Seq libraries were prepared according to the manufacturer protocol (10x Genomics, Pleasanton, CA).

RNA sequencing:

Article Title: Crosstalk between nonclassical monocytes and alveolar macrophages mediates transplant ischemia-reperfusion injury through classical monocyte recruitment.
Article Snippet: Cells were counted using acridine orange/propidium (AO/PI) and Cellometer K2 (Nexcelom), and cell viability exceeded 90%. .. Single cell 3’ RNA-Seq libraries were prepared using Chromium Single Cell V2 Reagent Kit and Controller (10x Genomics). .. Libraries were assessed for quality (TapeStation 4200, Agilent Technologies) and then sequenced on NextSeq 500 or HiSeq 4000 instruments (Illumina).



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